ABSTRACT
Objective To investigate the major effective Chinese materia medica inQuban Prescription for intervention in melanin synthesis.Methods 3×2 factorial design was used. The factors of investigation were Crataegi Fructus, Angelicae Sinensis Radix and Corni Fructus. Tyrosinase activity, melanin generation and auto-oxidation of dopa were determinated respectively; and the major effective Chinese materia medica ofQuban Prescription for intervention in melanin synthesis was analyzed by SPSS16.0 software.Results Tyrosinase activity was remarkably inhibited by Corni Fructus (P<0.05). Melanin generation was remarkably inhibited by Corni Fructus and Angelicae Sinensis Radix (P<0.01). Auto-oxidation of dopa was remarkably inhibited by Corni Fructus and Angelicae Sinensis Radix (P<0.05). Corni Fructus was in an absolute leading position.ConclusionCorni Fructus is the major effective Chinese materia medica in inhibiting tyrosinase activity, melanin generation and auto-oxidation of dopa in the prescription.
ABSTRACT
OBJECTIVE:To promote the work of clinical TCM pharmacists.METHODS:The problems faced by clinical TCM pharmacists were analyzed in respects of status quo of training in colleges and universities,status quo of hospital post training and status quo of continuing education.From the ability of post service,the ways to determine the duty orientation and improve the training quality of clinical TCM pharmacists were discussed theoretically.RESULTS & CONCLUSIONS:According to the nature of different types of medical institutions as TCM hospital,western medicine hospital,township community hospital and clinics,the responsibilities of clinical TCM pharmacists should be differentiated.TCM curriculum system of colleges and universities should be improved by adding social pharmaceutical education and TCM practical teaching.Based on the ability of post service,great importance should be attached to professional training of clinical TCM tablets after their employment.The theory,practice and research ability of clinical TCM talents should be improved by strengthening continuing education.The medical and pharmaceutical clinical TCM inter-disciplinary talent can be cultivated by improving rational drug use standard system.
ABSTRACT
Objective:To confi rm acidic extracts of Chinese materia medica having an inhibition to melanin synthesis. Methods: By L-DOPA oxidation method, the in vitro determination was studied to get tyrosinase activity’s inhibition of acidic extracts from 28 kinds of Chinese materia medica with high, middle and low concentration solution. Results: First, Acidic extracts from 28 kinds of Chinese materia medica at high concentration (25g/L) showed potent inhibitory action on tyrosinase activity. The highest inhibition rate was 96.50%, which was from the acidic extract of Fructus Mume. The next was fructus schizandrae with 85.33% inhibition rate. Compared with a 56.67% inhibition rate of 2 mmol/ml control sample arbutin, both Fructus Mume and fructus schizandrae had a large signifi cant deviation ( P